Hotspot

rpl32-trnL-UAG

SSC · 119326-120628 · 1303 bp

Back to Dactyladenia

Pi 0.00307
MarkerSeek score 38.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1303
variable_sites
6
pi
0.00307
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCGCGTTGAAAGCTTTTTC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1414-1428 bp 1.0 80.2
rpl32-trnL-UAG_p2 GACCGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1415-1429 bp 1.0 80.2
rpl32-trnL-UAG_p3 ACCGCGTTGAAAGCTTTTTC CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1415-1429 bp 1.0 80.2
rpl32-trnL-UAG_p4 GACCGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1416-1430 bp 1.0 80.2
rpl32-trnL-UAG_p5 ACCGCGTTGAAAGCTTTTTC GCATCATTAACCACCGGGAT 45.0 50.0 58.2 58.0 2023-2037 bp 1.0 79.7