Hotspot

petL-petG

LSC · 69700-69913 · 214 bp

Back to Dactyladenia

Pi 0.0109

MarkerSeek score 64.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 3
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTAGCCCCTTCATGCCTACT	CAACTGATCACCACGTCTGT	50.0	50.0	58.1	57.8	412-426 bp	1.0	81.1
petL-petG_p2	AGCCCCTTCATGCCTACTAT	CAACTGATCACCACGTCTGT	50.0	50.0	57.9	57.8	410-424 bp	1.0	81.0
petL-petG_p3	TTAGCCCCTTCATGCCTACT	CCAACTGATCACCACGTCTG	50.0	55.0	58.1	58.6	413-427 bp	1.0	77.8
petL-petG_p4	TTAGCCCCTTCATGCCTACT	AAGGTCCAACTGATCACCAC	50.0	50.0	58.1	57.4	418-432 bp	1.0	77.8
petL-petG_p5	TTAGCCCCTTCATGCCTACT	GTCCAACTGATCACCACGTC	50.0	55.0	58.1	58.6	415-429 bp	1.0	77.8