Hotspot

ndhC-trnV-UAC

LSC · 52929-54130 · 1202 bp

Back to Dactyladenia

Pi 0.00465

MarkerSeek score 66.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1202
variable_sites: 8
pi: 0.00465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATGGGGGATAAAACTCCGGA	GCATGTTGGGTCTTTGAAGC	50.0	50.0	58.1	57.9	1342-1400 bp	1.0	83.1
ndhC-trnV-UAC_p2	CTGGACCTTTGGTAATGGGG	GCATGTTGGGTCTTTGAAGC	55.0	50.0	57.9	57.9	1356-1414 bp	1.0	82.6
ndhC-trnV-UAC_p3	ATGGGGGATAAAACTCCGGA	GCAAGAGCCCAATGCAAAAA	50.0	45.0	58.1	58.1	1365-1423 bp	1.0	82.6
ndhC-trnV-UAC_p4	TCCGGAAATTCGAAATGCCA	GCATGTTGGGTCTTTGAAGC	45.0	50.0	58.2	57.9	1328-1386 bp	1.0	82.5
ndhC-trnV-UAC_p5	CTGGACCTTTGGTAATGGGG	GCAAGAGCCCAATGCAAAAA	55.0	45.0	57.9	58.1	1379-1437 bp	1.0	82.2