Hotspot

petN-psbM

LSC · 29539-30591 · 1053 bp

Back to Dactyladenia

Pi 0.0038

MarkerSeek score 61.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1053
variable_sites: 6
pi: 0.0038
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTATGGGGAAGAGGTGGACT	GCGCTGTTCATTCTTGTTCC	55.0	50.0	58.1	58.0	1148-1149 bp	1.0	79.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	58.2	58.0	1207-1208 bp	1.0	79.0
petN-psbM_p3	TATGGGGAAGAGGTGGACTC	GCGCTGTTCATTCTTGTTCC	55.0	50.0	57.8	58.0	1147-1148 bp	1.0	78.8
petN-psbM_p4	GTATGGGGAAGAGGTGGACT	ATTGCTACTGCGCTGTTCAT	55.0	45.0	58.1	58.3	1157-1158 bp	1.0	77.5
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1216-1217 bp	1.0	77.2