Hotspot

atpA-atpF

LSC · 11362-11426 · 65 bp

Back to Dactyladenia

Pi 0.0308

MarkerSeek score 70.40

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 3
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TATTGGCATGTTTGGAGCGA	55.0	45.0	58.1	57.9	218-220 bp	1.0	79.4
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	TATTGGCATGTTTGGAGCGA	55.0	45.0	58.1	57.9	216-218 bp	1.0	79.4
atpA-atpF_p3	TACTAATCTCGTCGGCTCGA	TATTGGCATGTTTGGAGCGA	50.0	45.0	57.8	57.9	136-138 bp	1.0	78.9
atpA-atpF_p4	TAATCTCGTCGGCTCGAATG	TATTGGCATGTTTGGAGCGA	50.0	45.0	57.6	57.9	133-135 bp	1.0	77.6
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	TATTGGCATGTTTGGAGCGA	50.0	45.0	58.4	57.9	209-211 bp	1.0	77.1