Hotspot

rpl16

LSC · 1359-2905 · 1547 bp

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Pi 0.0155
MarkerSeek score 57.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1547
variable_sites
36
pi
0.0155
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCATTTGATATTGATAGAATTGCT TCCCAAAGACCTCTTTCCTT 25.0 45.0 52.6 55.9 1603 bp 0.333333 13.3
rpl16_p2 TCATTTGATATTGATAGAATTGCT TCCCAAAGACCTCTTTCCTTTG 25.0 45.5 52.6 58.2 1603 bp 0.333333 13.3
rpl16_p3 TCATTTGATATTGATAGAATTGCT TCCCAAAGACCTCTTTCCTTT 25.0 42.9 52.6 56.7 1603 bp 0.333333 13.3
rpl16_p4 TCATTTGATATTGATAGAATTGCT CCCAAAGACCTCTTTCCTTTG 25.0 47.6 52.6 56.7 1602 bp 0.333333 13.3
rpl16_p5 TCATTTGATATTGATAGAATTGCT AGACCTCTTTCCTTTGGTTCT 25.0 42.9 52.6 56.5 1597 bp 0.333333 13.3