Hotspot

atpB-rbcL

LSC · 75301-76089 · 789 bp

Back to Cytisus

Pi 0.0221

MarkerSeek score 63.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 24
pi: 0.0221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACGCAGAAAACTTGGGATCA	GGCTTTGAACCCAACACTTG	45.0	50.0	57.7	57.8	877-880 bp	1.0	79.3
atpB-rbcL_p2	ACGCAGAAAACTTGGGATCA	GCTTTGAACCCAACACTTGC	45.0	50.0	57.7	58.4	876-879 bp	1.0	77.7
atpB-rbcL_p3	ACGCAGAAAACTTGGGATCA	TTAACACCGGCTTTGAACCC	45.0	50.0	57.7	58.7	885-888 bp	1.0	75.6
atpB-rbcL_p4	TGAAACGCAGAAAACTTGGG	GGCTTTGAACCCAACACTTG	45.0	50.0	57.1	57.8	881-884 bp	1.0	74.6
atpB-rbcL_p5	CGCAGAAAACTTGGGATCAG	GGCTTTGAACCCAACACTTG	50.0	50.0	57.1	57.8	876-879 bp	1.0	74.2