Hotspot

trnF-ndhJ

LSC · 69606-70209 · 604 bp

Back to Cytisus

Pi 0.0304

MarkerSeek score 69.00

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 27
pi: 0.0304
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.0	57.9	726-768 bp	1.0	85.3
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	690-732 bp	1.0	85.2
trnF-ndhJ_p3	AGAATGGTCGGGATAGCTCA	TTGGATAGGATGGCCTTTGC	50.0	50.0	57.9	57.9	735-777 bp	1.0	84.5
trnF-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	TGGCATTCCATTGCTGTCAT	55.0	45.0	58.0	58.1	1028-1070 bp	1.0	83.2
trnF-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AGGATTGGCATTCCATTGCT	55.0	45.0	58.0	57.8	1033-1075 bp	1.0	83.0