Hotspot

trnG-trnS

LSC · 65845-66666 · 822 bp

Back to Cytisus

Pi 0.0294

MarkerSeek score 76.80

Primer available yes

Label trnG-trnS

Feature Metadata

label_name: trnG-trnS
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 35
pi: 0.0294
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnS_p1	TGAATCAAACGAGGGATCCC	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.3	58.0	903-934 bp	1.0	81.1
trnG-trnS_p2	TGAATCAAACGAGGGATCCC	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.3	58.0	902-933 bp	1.0	81.1
trnG-trnS_p3	TGAATCAAACGAGGGATCCC	AGCAATCCGACGCTTTAGTC	50.0	50.0	57.3	58.4	905-936 bp	1.0	78.2
trnG-trnS_p4	TGAATCAAACGAGGGATCCC	GTCAGTAACGCGGAAAGAGA	50.0	50.0	57.3	57.7	965-996 bp	1.0	77.9
trnG-trnS_p5	TGAATCAAACGAGGGATCCC	CAGTAACGCGGAAAGAGAGG	50.0	55.0	57.3	58.4	963-994 bp	1.0	77.7