Hotspot

trnR-trnG

LSC · 64910-65059 · 150 bp

Back to Cytisus

Pi 0.0578

MarkerSeek score 74.10

Primer available yes

Label trnR-trnG

Feature Metadata

label_name: trnR-trnG
feature_type: igs
region: LSC
length_bp: 150
variable_sites: 13
pi: 0.0578
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnG_p1	AGAAGACCTCTGTCCTATCCA	GGTTCGATTCCCGCTACC	47.6	61.1	56.9	57.6	208-374 bp	1.0	48.0
trnR-trnG_p2	AGAAGACCTCTGTCCTATCCA	GTTCGATTCCCGCTACCC	47.6	61.1	56.9	57.6	207-373 bp	1.0	48.0
trnR-trnG_p3	AGAAGACCTCTGTCCTATCCA	GGGTTCGATTCCCGCTAC	47.6	61.1	56.9	57.6	209-375 bp	1.0	47.9
trnR-trnG_p4	AGGTTTAGAAGACCTCTGTCCT	GGTTCGATTCCCGCTACC	45.5	61.1	57.9	57.6	214-380 bp	1.0	47.2
trnR-trnG_p5	AGGTTTAGAAGACCTCTGTCCT	GTTCGATTCCCGCTACCC	45.5	61.1	57.9	57.6	213-379 bp	1.0	47.2