Hotspot

atpI-atpH

LSC · 59944-61280 · 1337 bp

Back to Cytisus

Pi 0.02

MarkerSeek score 68.60

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1337
variable_sites: 35
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CCGGTCATGTTCCTTGGATT	CTCTACAGCTTGTCCTGCAG	50.0	55.0	57.9	58.0	1069-1550 bp	1.0	84.9
atpI-atpH_p2	CCGGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.9	58.0	990-1471 bp	1.0	84.8
atpI-atpH_p3	CCGGTCATGTTCCTTGGATT	GTCCAATAGAAGCAAGCCCA	50.0	50.0	57.9	57.9	1028-1509 bp	1.0	84.0
atpI-atpH_p4	CCGGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.9	58.2	991-1472 bp	1.0	83.6
atpI-atpH_p5	TACCGGTCATGTTCCTTGGA	CTCTACAGCTTGTCCTGCAG	50.0	55.0	58.4	58.0	1071-1552 bp	1.0	83.1