Hotspot

trnfM-trnG

LSC · 38486-38612 · 127 bp

Back to Cytisus

Pi 0.0394

MarkerSeek score 69.80

Primer available yes

Label trnfM-trnG

Feature Metadata

label_name: trnfM-trnG
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 7
pi: 0.0394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-trnG_p1	CTTGAGGTCACGGGTTCAAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	58.0	57.8	204-214 bp	1.0	81.4
trnfM-trnG_p2	GGGTAGAGCAGTTTGGTAGC	TCTTTGCCAAGGAGAAGACG	55.0	50.0	58.0	57.8	240-250 bp	1.0	81.1
trnfM-trnG_p3	TGAGGTCACGGGTTCAAATC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	202-212 bp	1.0	80.3
trnfM-trnG_p4	TAGCTCGCAAGGCTCATAAC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	224-234 bp	1.0	79.7
trnfM-trnG_p5	GTAGCTCGCAAGGCTCATAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	225-235 bp	1.0	79.6