Hotspot

petG-petL

LSC · 18431-18593 · 163 bp

Back to Cytisus

Pi 0.0327

MarkerSeek score 66.50

Primer available yes

Label petG-petL

Feature Metadata

label_name: petG-petL
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 8
pi: 0.0327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-petL_p1	AAGGTCCAACTGATCACCAC	TGGTTTTCTACTAGCGGCTT	50.0	45.0	57.4	56.9	347-348 bp	1.0	68.1
petG-petL_p2	AAGGTCCAACTGATCACCAC	TTGGTTTTCTACTAGCGGCT	50.0	45.0	57.4	56.9	348-349 bp	1.0	68.1
petG-petL_p3	GTCCAACTGATCACCACGTC	TGGTTTTCTACTAGCGGCTT	55.0	45.0	58.6	56.9	344-345 bp	1.0	68.1
petG-petL_p4	GTCCAACTGATCACCACGTC	TTGGTTTTCTACTAGCGGCT	55.0	45.0	58.6	56.9	345-346 bp	1.0	68.1
petG-petL_p5	AAGGTCCAACTGATCACCAC	TGGTTTTCTACTAGCGGCTTT	50.0	42.9	57.4	57.6	347-348 bp	1.0	66.0