Hotspot

rpl33-psaJ

LSC · 16916-17400 · 485 bp

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Pi 0.033
MarkerSeek score 64.80
Primer available yes
Label rpl33-psaJ

Feature Metadata

label_name
rpl33-psaJ
feature_type
igs
region
LSC
length_bp
485
variable_sites
24
pi
0.033
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-psaJ_p1 TATCTTTACCCTTGGCCAT GATGCATTGATATTCCCGTT 42.1 40.0 53.0 53.8 537-554 bp 1.0 44.1
rpl33-psaJ_p2 TATCTTTACCCTTGGCCAT GATGCATTGATATTCCCGTTT 42.1 38.1 53.0 54.7 537-554 bp 1.0 44.1
rpl33-psaJ_p3 TATCTTTACCCTTGGCCAT TGCATTGATATTCCCGTTTT 42.1 35.0 53.0 53.5 535-552 bp 1.0 44.1
rpl33-psaJ_p4 TATCTTTACCCTTGGCCAT GATGCATTGATATTCCCGTTTT 42.1 36.4 53.0 55.5 537-554 bp 1.0 44.1
rpl33-psaJ_p5 TATCTTTACCCTTGGCCAT TGCATTGATATTCCCGTTTTT 42.1 33.3 53.0 54.4 535-552 bp 1.0 44.1