Hotspot

rpoA-petD

LSC · 6463-6684 · 222 bp

Back to Cytisus

Pi 0.0581

MarkerSeek score 76.30

Primer available yes

Label rpoA-petD

Feature Metadata

label_name: rpoA-petD
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 19
pi: 0.0581
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-petD_p1	GAAGCCTCCCGGAATTTGAT	TAATGGTTTCGGTACCTGCG	50.0	50.0	57.9	58.0	750-755 bp	1.0	83.7
rpoA-petD_p2	GAAGCCTCCCGGAATTTGAT	GGTTGGGTATTGGAGCAACA	50.0	50.0	57.9	58.1	624-629 bp	1.0	83.7
rpoA-petD_p3	GAAGCCTCCCGGAATTTGAT	TGGTTGGGTATTGGAGCAAC	50.0	50.0	57.9	58.1	625-630 bp	1.0	83.7
rpoA-petD_p4	GAAGCCTCCCGGAATTTGAT	GTTTGATTCGTGCTTGAGCC	50.0	50.0	57.9	58.0	1128-1133 bp	1.0	82.3
rpoA-petD_p5	GAAGCCTCCCGGAATTTGAT	GTGCTTGAGCCGTATGATGA	50.0	50.0	57.9	58.1	1119-1124 bp	1.0	82.0