Hotspot

rpl36-rps11

LSC · 4654-4974 · 321 bp

Back to Cytisus

Pi 0.0304

MarkerSeek score 67.80

Primer available yes

Label rpl36-rps11

Feature Metadata

label_name: rpl36-rps11
feature_type: igs
region: LSC
length_bp: 321
variable_sites: 14
pi: 0.0304
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps11_p1	AAAATAAGGGCCTCTGCTCG	TCCATTTTTACGCGAACCCA	50.0	45.0	58.0	58.1	458-476 bp	1.0	84.6
rpl36-rps11_p2	AAAATAAGGGCCTCTGCTCG	CGTCCATTTTTACGCGAACC	50.0	50.0	58.0	58.1	460-478 bp	1.0	84.4
rpl36-rps11_p3	AAAATAAGGGCCTCTGCTCG	TACGCGAACCCATTTTGAGT	50.0	45.0	58.0	57.8	450-468 bp	1.0	84.2
rpl36-rps11_p4	AAAATAAGGGCCTCTGCTCG	GTCCATTTTTACGCGAACCC	50.0	50.0	58.0	57.7	459-477 bp	1.0	83.4
rpl36-rps11_p5	ATAAGGGCCTCTGCTCGTAA	TCCATTTTTACGCGAACCCA	50.0	45.0	58.2	58.1	455-473 bp	1.0	83.3