Hotspot

trnP-GGG-trnL-UAG

SSC · 109860-110127 · 268 bp

Back to Cyrtomium

Pi 0.0449
MarkerSeek score 69.00
Primer available yes
Label trnP-GGG-trnL-UAG

Feature Metadata

label_name
trnP-GGG-trnL-UAG
feature_type
igs
region
SSC
length_bp
268
variable_sites
22
pi
0.0449
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-trnL-UAG_p1 GATTCGAACCTGTGACCTCC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 363-382 bp 1.0 88.5
trnP-GGG-trnL-UAG_p2 GATTCGAACCTGTGACCTCC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 361-380 bp 1.0 88.5
trnP-GGG-trnL-UAG_p3 GGATTCGAACCTGTGACCTC CTCTGGCACTGCTTCCTAAG 55.0 55.0 58.0 58.0 364-383 bp 1.0 88.5
trnP-GGG-trnL-UAG_p4 GGATTCGAACCTGTGACCTC CTGGCACTGCTTCCTAAGAG 55.0 55.0 58.0 58.0 362-381 bp 1.0 88.5
trnP-GGG-trnL-UAG_p5 TCCACTCGGAATAGCAGGAT CTGGCACTGCTTCCTAAGAG 50.0 55.0 57.9 58.0 378-397 bp 1.0 87.7