Hotspot

trnM-CAU-atpE

LSC · 49604-49760 · 157 bp

Back to Cyrtomium

Pi 0.0378

MarkerSeek score 57.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 12
pi: 0.0378
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	AATTACTGGCGGCCTACTTG	GCTAGAAGCCTCAAATGTTGC	50.0	47.6	58.0	57.9	266-267 bp	1.0	74.2
trnM-CAU-atpE_p2	GCGAGAGTCATTGGTTCGAA	GCTAGAAGCCTCAAATGTTGC	50.0	47.6	58.3	57.9	216-217 bp	1.0	73.0
trnM-CAU-atpE_p3	TCATACGGCGAGAGTCATTG	GCTAGAAGCCTCAAATGTTGC	50.0	47.6	57.5	57.9	223-224 bp	1.0	71.2
trnM-CAU-atpE_p4	CATACGGCGAGAGTCATTGG	GCTAGAAGCCTCAAATGTTGC	55.0	47.6	58.5	57.9	222-223 bp	1.0	71.1
trnM-CAU-atpE_p5	CTTTCATACGGCGAGAGTCA	GCTAGAAGCCTCAAATGTTGC	50.0	47.6	57.4	57.9	226-227 bp	1.0	70.6