Hotspot

trnS-UGA-psbC

LSC · 31170-31456 · 287 bp

Back to Cyrtomium

Pi 0.023

MarkerSeek score 60.40

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 17
pi: 0.023
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GTCTTGAAAACTGGCATGGC	CGATACCGAACCCGTTCTTT	50.0	50.0	57.9	58.0	377-390 bp	1.0	84.8
trnS-UGA-psbC_p2	GTCTTGAAAACTGGCATGGC	AAGGAATTGACCGCGATACC	50.0	50.0	57.9	58.1	390-403 bp	1.0	84.3
trnS-UGA-psbC_p3	GGGTTCGAATCCCTCTCTCT	CGATACCGAACCCGTTCTTT	55.0	50.0	58.2	58.0	341-354 bp	1.0	84.2
trnS-UGA-psbC_p4	GTCTTGAAAACTGGCATGGC	ACCCGTTCTTTCCATGACAC	50.0	50.0	57.9	58.1	368-381 bp	1.0	84.1
trnS-UGA-psbC_p5	GTCTTGAAAACTGGCATGGC	GCAGCTGGGTTTGAAAAAGG	50.0	50.0	57.9	58.1	406-419 bp	1.0	83.4