Hotspot

ycf12-trnG-UCC

LSC · 8609-8776 · 168 bp

Back to Cyrtomium

Pi 0.0295

MarkerSeek score 57.90

Primer available yes

Label ycf12-trnG-UCC

Feature Metadata

label_name: ycf12-trnG-UCC
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 10
pi: 0.0295
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf12-trnG-UCC_p1	TCGGGGCCATTAGTAATTGC	AGACGAGTCGCACTTTTACC	50.0	50.0	58.0	57.9	252-255 bp	1.0	83.7
ycf12-trnG-UCC_p2	TCGGGGCCATTAGTAATTGC	CGAGTCGCACTTTTACCACT	50.0	50.0	58.0	58.2	249-252 bp	1.0	82.5
ycf12-trnG-UCC_p3	TCGGGGCCATTAGTAATTGC	GACGAGTCGCACTTTTACCA	50.0	50.0	58.0	58.2	251-254 bp	1.0	82.4
ycf12-trnG-UCC_p4	GCATCGGGGCCATTAGTAAT	AGACGAGTCGCACTTTTACC	50.0	50.0	57.8	57.9	255-258 bp	1.0	82.3
ycf12-trnG-UCC_p5	GTGCAACTTGCTATCTTGGC	AGACGAGTCGCACTTTTACC	50.0	50.0	57.7	57.9	285-288 bp	1.0	81.3