Hotspot

psbK-psbI

LSC · 7079-7630 · 552 bp

Back to Cyrtomium

Pi 0.0206

MarkerSeek score 54.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 26
pi: 0.0206
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGCAGTTAGCTTCCGGTAA	ATCTAATCCCTACGTCCGGG	52.6	55.0	57.6	58.1	682-693 bp	1.0	71.4
psbK-psbI_p2	CGCAGTTAGCTTCCGGTAA	GGGTTTCGTCCAGGATCATT	52.6	50.0	57.6	57.6	664-675 bp	1.0	68.8
psbK-psbI_p3	TTTGGCAAGCCGCAGTTAG	ATCTAATCCCTACGTCCGGG	52.6	55.0	59.0	58.1	692-703 bp	1.0	66.5
psbK-psbI_p4	CCGCAGTTAGCTTCCGGTAA	ATCTAATCCCTACGTCCGGG	55.0	55.0	60.1	58.1	683-694 bp	1.0	66.0
psbK-psbI_p5	TTTGGCAAGCCGCAGTTA	ATCTAATCCCTACGTCCGGG	50.0	55.0	57.8	58.1	692-703 bp	1.0	65.5