Hotspot

ycf1

SSC · 127918-133386 · 5469 bp

Back to Cyrtanthus

Pi 0.00731

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 40
pi: 0.00731
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCTTGGATCTATCAGTCT	CGGGAGCAGACATTAAAAAGG	45.0	47.6	54.3	57.2	184 bp	1.0	42.2
ycf1_p2	AGCAATTTCCCTTGGATCTATCA	CGGGAGCAGACATTAAAAAGG	39.1	47.6	57.6	57.2	191 bp	1.0	42.1
ycf1_p3	AGCAATTTCCCTTGGATCTATCA	CACGGGAGCAGACATTAAAA	39.1	45.0	57.6	56.1	193 bp	1.0	42.1
ycf1_p4	GCAATTTCCCTTGGATCTATCAG	CGGGAGCAGACATTAAAAAGG	43.5	47.6	57.3	57.2	190 bp	1.0	42.1
ycf1_p5	AGCAATTTCCCTTGGATCTATCA	CACGGGAGCAGACATTAAAAAG	39.1	45.5	57.6	57.9	193 bp	1.0	42.1