Hotspot

rpl32-trnL-UAG

SSC · 117238-118058 · 821 bp

Back to Cyrtanthus

Pi 0.0159
MarkerSeek score 68.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
821
variable_sites
13
pi
0.0159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 933-935 bp 1.0 70.7
rpl32-trnL-UAG_p2 GCAAAGTCAGTTTCTACCGGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 932-934 bp 1.0 70.7
rpl32-trnL-UAG_p3 GCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.7 57.9 932-934 bp 1.0 70.5
rpl32-trnL-UAG_p4 AGCAAAGTCAGTTTCTACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 926-928 bp 1.0 70.2
rpl32-trnL-UAG_p5 GCAAAGTCAGTTTCTACCGGA AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 925-927 bp 1.0 70.2