Hotspot

rpl33-rps18

LSC · 71129-71317 · 189 bp

Back to Cyrtanthus

Pi 0.037

MarkerSeek score 65.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 7
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TGTCGCTATTGTCACAAGCA	CAATTCGATCCCCCGATTCA	45.0	50.0	57.8	57.7	322-351 bp	1.0	78.3
rpl33-rps18_p2	TGTCGCTATTGTCACAAGCA	TCGATCCCCCGATTCAATTG	45.0	50.0	57.8	57.7	318-347 bp	1.0	78.3
rpl33-rps18_p3	TGTCGCTATTGTCACAAGCA	TCAATTCGATCCCCCGATTC	45.0	50.0	57.8	57.5	323-352 bp	1.0	76.0
rpl33-rps18_p4	TGTCGCTATTGTCACAAGCA	GGGAAAACGCCTACGAAAAG	45.0	50.0	57.8	57.4	297-326 bp	1.0	75.6
rpl33-rps18_p5	CGACACAATACACCCAGTCG	TCGATCCCCCGATTCAATTG	55.0	50.0	58.7	57.7	357-386 bp	1.0	74.2