Hotspot

petN-psbM

LSC · 30142-31183 · 1042 bp

Back to Cyrtanthus

Pi 0.0158

MarkerSeek score 55.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 16
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ACCCAGAGAATACCGGTCAT	45.0	50.0	58.0	57.8	1577-1598 bp	1.0	81.0
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	ATGACCCAGAGAATACCGGT	45.0	50.0	58.0	57.8	1580-1601 bp	1.0	81.0
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	AAGCAACGCCCCTTTCTTAT	45.0	45.0	58.0	57.8	1677-1698 bp	1.0	80.5
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	AGCAACGCCCCTTTCTTATT	45.0	45.0	58.0	57.8	1676-1697 bp	1.0	80.5
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	ACCCAGAGAATACCGGTCAT	55.0	50.0	58.1	57.8	1525-1546 bp	1.0	80.1