Hotspot

trnS-GCU-trnG-GCC

LSC · 8644-9932 · 1289 bp

Back to Cyrtanthus

Pi 0.0234

MarkerSeek score 64.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 29
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	45.0	40.9	58.0	57.3	1366-1622 bp	1.0	62.3
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	45.0	40.9	58.0	57.0	1367-1623 bp	1.0	60.2
trnS-GCU-trnG-GCC_p3	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1352-1608 bp	1.0	59.3
trnS-GCU-trnG-GCC_p4	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1347-1603 bp	1.0	58.5
trnS-GCU-trnG-GCC_p5	GCTTTAGTCCACTCAGCCAT	TCGAACGAATCACACTTTTACC	50.0	40.9	57.6	57.0	1353-1609 bp	1.0	57.3