Hotspot

psbA-trnK-UUU

LSC · 1149-1392 · 244 bp

Back to Cyrtanthus

Pi 0.0287

MarkerSeek score 69.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 7
pi: 0.0287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAAACACCGAACCATCCGAT	GTTTGGGGAGGGGTCTTTTT	45.0	50.0	57.8	57.9	441-457 bp	1.0	78.9
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	GTTTGGGGAGGGGTCTTTTT	45.0	50.0	57.8	57.9	420-436 bp	1.0	78.6
psbA-trnK-UUU_p3	GCTTGTACTTTCGCGTCTCT	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	58.3	57.9	370-386 bp	1.0	78.3
psbA-trnK-UUU_p4	CACCGAACCATCCGATGTAA	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	57.7	57.9	437-453 bp	1.0	78.1
psbA-trnK-UUU_p5	GTAAAGACGGTTTTCGGTGC	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	57.7	57.9	421-437 bp	1.0	78.1