Hotspot

ndhB-trnL-CAA

IRa · 188957-189553 · 597 bp

Back to Cypripedium

Pi 0.04

MarkerSeek score 83.50

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 597
variable_sites: 84
pi: 0.04
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	617-1027 bp	1.0	91.5
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	615-1025 bp	1.0	91.5
ndhB-trnL-CAA_p3	CCCGATTCTTGCAATTGCTC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.8	58.0	626-1036 bp	1.0	90.3
ndhB-trnL-CAA_p4	CCCGATTCTTGCAATTGCTC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.8	58.0	624-1034 bp	1.0	90.3
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	612-1022 bp	1.0	90.0