Hotspot

ndhF-rpl32

SSC · 149662-151140 · 1479 bp

Back to Cypripedium

Pi 0.226
MarkerSeek score 79.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1479
variable_sites
800
pi
0.226
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.90
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAGCGGAAAAAGGGGTATT CTTTTTGACTGTCCGGTGGA 50.0 50.0 57.9 58.0 1525-2679 bp 0.777778 81.7
ndhF-rpl32_p2 ACATAGTAACTGGGAGCGGA CTTTTTGACTGTCCGGTGGA 50.0 50.0 57.8 58.0 1537-2691 bp 0.777778 81.6
ndhF-rpl32_p3 AGCAACTGCACCGACAAATA CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 2193-2691 bp 0.444444 68.6
ndhF-rpl32_p4 ATTTAGCAACTGCACCGACA CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 2197-2695 bp 0.444444 68.6
ndhF-rpl32_p5 TAGCAACTGCACCGACAAAT CTTTTTGACTGTCCGGTGGA 45.0 50.0 58.1 58.0 2194-2692 bp 0.444444 68.6