Hotspot

trnP-UGG-psaJ

LSC · 89053-90538 · 1486 bp

Back to Cypripedium

Pi 0.276

MarkerSeek score 86.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 1486
variable_sites: 975
pi: 0.276
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	1575-2717 bp	0.222222	55.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	1574-2716 bp	0.222222	55.2
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	1586-2728 bp	0.222222	55.2
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	1585-2727 bp	0.222222	55.2
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	1588-2730 bp	0.222222	55.2