Hotspot

pafII-cemA

LSC · 81930-82865 · 936 bp

Back to Cypripedium

Pi 0.0834

MarkerSeek score 83.10

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 936
variable_sites: 181
pi: 0.0834
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	CCGTATTTCCGTCTCAGCAA	TATGGGAGGGAAGCAAATGC	50.0	50.0	58.0	57.9	1298-2679 bp	1.0	91.4
pafII-cemA_p2	GGAGGTGAGAGAGGGTCTTT	TATGGGAGGGAAGCAAATGC	55.0	50.0	58.1	57.9	1057-2438 bp	1.0	91.0
pafII-cemA_p3	TTTCGTTGGGGATTTCCTGG	TATGGGAGGGAAGCAAATGC	50.0	50.0	58.1	57.9	1134-2515 bp	1.0	90.8
pafII-cemA_p4	CCGTATTTCCGTCTCAGCAA	GTGTGTCCGAGTACTGCTTT	50.0	50.0	58.0	57.8	1513-2882 bp	1.0	90.8
pafII-cemA_p5	TTCTATGGGATCGCAGGTCT	TATGGGAGGGAAGCAAATGC	50.0	50.0	57.9	57.9	1245-2626 bp	1.0	90.7