Hotspot

atpB-rbcL

LSC · 71577-75632 · 4056 bp

Back to Cypripedium

Pi 0.207

MarkerSeek score 75.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 4056
variable_sites: 1901
pi: 0.207
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TCTTTCTATTGTATTGTGTATTGT	ATATGGCGCAACCCAATCTT	25.0	45.0	52.2	57.9	2380 bp	0.111111	4.4
atpB-rbcL_p2	TCTTTCTATTGTATTGTGTATTGT	CGCAACCCAATCTTCGTTTT	25.0	45.0	52.2	57.6	2374 bp	0.111111	4.4
atpB-rbcL_p3	TCTTTCTATTGTATTGTGTATTGT	TATGGCGCAACCCAATCTTC	25.0	50.0	52.2	58.6	2379 bp	0.111111	4.4
atpB-rbcL_p4	TCTTTCTATTGTATTGTGTATTGT	ACAACGACAAGGTCTACTCG	25.0	50.0	52.2	57.3	2682 bp	0.111111	4.4
atpB-rbcL_p5	TCTTTCTATTGTATTGTGTATTGT	AAGTCCCTCCCTACAACTCA	25.0	50.0	52.2	57.3	2718 bp	0.111111	4.4