Hotspot

trnT-UGU-trnL-UAA

LSC · 57960-59363 · 1404 bp

Back to Cypripedium

Pi 0.0665
MarkerSeek score 83.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1404
variable_sites
241
pi
0.0665
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.91
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 623-1466 bp 0.777778 82.0
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 631-1474 bp 0.777778 81.5
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 655-1498 bp 0.777778 81.2
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 663-1506 bp 0.777778 80.6
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG ATCCAATCAAGTCCGTAGCG 55.0 50.0 57.9 57.8 646-1489 bp 0.777778 80.2