Hotspot

trnE-UUC-trnT-GGU

LSC · 41266-42752 · 1487 bp

Back to Cypripedium

Pi 0.106

MarkerSeek score 89.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1487
variable_sites: 390
pi: 0.106
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1410-2067 bp	0.666667	77.3
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	1375-2032 bp	0.666667	76.5
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1388-2045 bp	0.666667	76.4
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	1407-2064 bp	0.666667	76.1
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	1353-2010 bp	0.666667	75.6