Hotspot

psbM-trnD-GUC

LSC · 39763-40521 · 759 bp

Back to Cypripedium

Pi 0.0633

MarkerSeek score 85.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 115
pi: 0.0633
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGCAAGAATATTGACTTCCA	AGTTCAATCGGTCAGAGCAC	40.0	50.0	53.7	57.9	846-1417 bp	1.0	57.0
psbM-trnD-GUC_p2	CGCAAGAATATTGACTTCCAT	AGTTCAATCGGTCAGAGCAC	38.1	50.0	54.4	57.9	846-1417 bp	1.0	54.1
psbM-trnD-GUC_p3	CGCAAGAATATTGACTTCCA	GTTCAATCGGTCAGAGCACC	40.0	55.0	53.7	58.9	845-1416 bp	1.0	52.0
psbM-trnD-GUC_p4	CGCAAGAATATTGACTTCCA	GTAGTTCAATCGGTCAGAGCA	40.0	47.6	53.7	57.8	848-1419 bp	1.0	52.0
psbM-trnD-GUC_p5	CGCAAGAATATTGACTTCCA	TAGTTCAATCGGTCAGAGCAC	40.0	47.6	53.7	57.8	847-1418 bp	1.0	52.0