Hotspot

trnS-GCU-trnG-UCC

LSC · 11673-14093 · 2421 bp

Back to Cypripedium

Pi 0.224
MarkerSeek score 78.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
2421
variable_sites
1342
pi
0.224
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TCTACTCGCGGGATCCTAAG 50.0 55.0 58.0 58.1 2364-2947 bp 0.555556 73.3
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TCTACTCGCGGGATCCTAAG 50.0 55.0 58.0 58.1 2363-2946 bp 0.555556 73.3
trnS-GCU-trnG-UCC_p3 CTTTAGTCCACTCAGCCACC TCTACTCGCGGGATCCTAAG 55.0 55.0 57.9 58.1 2354-2937 bp 0.555556 72.6
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA ATTTCTACTCGCGGGATCCT 50.0 50.0 58.0 58.3 2367-2950 bp 0.555556 71.8
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC ATTTCTACTCGCGGGATCCT 50.0 50.0 58.0 58.3 2366-2949 bp 0.555556 71.8