Hotspot

trnK-UUU-rps16

LSC · 6811-7547 · 737 bp

Back to Cypripedium

Pi 0.0569
MarkerSeek score 83.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
737
variable_sites
125
pi
0.0569
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGCCGAGTACTCTACCGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 894-1000 bp 1.0 89.5
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1368-1774 bp 0.555556 68.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1367-1773 bp 0.555556 68.3
trnK-UUU-rps16_p4 TCACGATTTCACGACATGCT GCAAAGACAACGAATTCCGG 45.0 50.0 57.9 58.0 1500-1514 bp 0.444444 58.6
trnK-UUU-rps16_p5 TCACGATTTCACGACATGCT TCGATCCCGAAGAGAAGGAA 45.0 50.0 57.9 57.9 1061-1076 bp 0.444444 57.8