Hotspot

rps15-trnN-GUU

SSC · 128319-134583 · 6265 bp

Back to Cyphostemma

Pi 0.0173
MarkerSeek score 50.00
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6265
variable_sites
108
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CCACTAACTCTCTACGGAAATCC TGGCGACTTCGAAGAGTTTC 47.8 50.0 58.1 58.2 215 bp 1.0 57.1
rps15-trnN-GUU_p2 CGGTTGTTGTATATACTCGAGC TGGCGACTTCGAAGAGTTTC 45.5 50.0 56.8 58.2 193 bp 1.0 56.3
rps15-trnN-GUU_p3 CCACTAACTCTCTACGGAAATCC ATGGCGACTTCGAAGAGTTT 47.8 45.0 58.1 57.5 216 bp 1.0 55.2
rps15-trnN-GUU_p4 CCACTAACTCTCTACGGAAATCC AATGGCGACTTCGAAGAGTT 47.8 45.0 58.1 57.5 217 bp 1.0 55.1
rps15-trnN-GUU_p5 CGGTTGTTGTATATACTCGAGCA TGGCGACTTCGAAGAGTTTC 43.5 50.0 58.4 58.2 193 bp 1.0 54.8