Hotspot

ndhF-rpl32

SSC · 116541-117545 · 1005 bp

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Pi 0.033
MarkerSeek score 67.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1005
variable_sites
33
pi
0.033
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGAATTGGAAGGGGAAGGA TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.9 52.0 1092-1097 bp 1.0 42.8
ndhF-rpl32_p2 AGGAATTGGAAGGGGAAGGA TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.9 52.0 1093-1098 bp 1.0 42.8
ndhF-rpl32_p3 AGGAATTGGAAGGGGAAGGA TTTTTGACATAGAAGTACGTTT 50.0 27.3 57.9 52.0 1094-1099 bp 1.0 42.8
ndhF-rpl32_p4 AGAAGAAGCCCCACTCCTAG TTTGACATAGAAGTACGTTTTT 55.0 27.3 58.1 52.0 1118-1123 bp 1.0 42.7
ndhF-rpl32_p5 AGAAGAAGCCCCACTCCTAG TTTTGACATAGAAGTACGTTTT 55.0 27.3 58.1 52.0 1119-1124 bp 1.0 42.7