Hotspot

rpl16-rps3

LSC · 85354-86654 · 1301 bp

Back to Cyphostemma

Pi 0.0162
MarkerSeek score 56.10
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1301
variable_sites
21
pi
0.0162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 GCCCCGCTTCTATTTGTCTA TGGATATTTGTAGACGAGGA 50.0 40.0 57.4 52.9 1428-1429 bp 1.0 41.5
rpl16-rps3_p2 TGTGATCCAAGCAGGTTCAA TGGATATTTGTAGACGAGGA 45.0 40.0 57.3 52.9 1406-1407 bp 1.0 41.5
rpl16-rps3_p3 GCCCCGCTTCTATTTGTCTA TGGATATTTGTAGACGAGGAA 50.0 38.1 57.4 53.8 1428-1429 bp 1.0 41.5
rpl16-rps3_p4 GCCCCGCTTCTATTTGTCTA TTGGATATTTGTAGACGAGGA 50.0 38.1 57.4 53.8 1429-1430 bp 1.0 41.5
rpl16-rps3_p5 TGTGATCCAAGCAGGTTCAA TGGATATTTGTAGACGAGGAA 45.0 38.1 57.3 53.8 1406-1407 bp 1.0 41.5