Hotspot

rpl20-rps12

LSC · 72764-73565 · 802 bp

Back to Cyphostemma

Pi 0.00511
MarkerSeek score 63.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
802
variable_sites
4
pi
0.00511
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGGGCTCCTCGAAA CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 900-918 bp 1.0 80.1
rpl20-rps12_p2 TTGAATGGGCTCCTCGAAAG CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 899-917 bp 1.0 80.1
rpl20-rps12_p3 GCTCCTCGAAAGCTTGATGT CGCCGAGGAACATGTACTAG 50.0 55.0 58.3 57.9 891-909 bp 1.0 78.9
rpl20-rps12_p4 CTTGAATGGGCTCCTCGAAA GCCGAGGAACATGTACTAGG 50.0 55.0 57.9 57.5 899-917 bp 1.0 77.0
rpl20-rps12_p5 TTGAATGGGCTCCTCGAAAG GCCGAGGAACATGTACTAGG 50.0 55.0 57.9 57.5 898-916 bp 1.0 77.0