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psbE-petL

LSC · 68305-69594 · 1290 bp

Back to Cyphostemma

Pi 0.0087

MarkerSeek score 65.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1290
variable_sites: 11
pi: 0.0087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1347-1358 bp	1.0	80.9
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1349-1360 bp	1.0	80.8
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1433-1444 bp	1.0	80.6
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.2	58.0	1435-1446 bp	1.0	80.6
psbE-petL_p5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.2	58.0	1438-1449 bp	1.0	80.5