Hotspot

accD-psaI

LSC · 62673-63372 · 700 bp

Back to Cyphostemma

Pi 0.0146

MarkerSeek score 66.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 10
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	840-849 bp	1.0	68.5
accD-psaI_p2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	839-848 bp	1.0	68.5
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	840-849 bp	1.0	66.7
accD-psaI_p4	TCAGTTTCACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.0	58.0	816-825 bp	1.0	66.5
accD-psaI_p5	CAGTTTCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.0	58.0	815-824 bp	1.0	66.5