Pi
0.00944
MarkerSeek score
66.50
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAAAAGCCAGCTATCGGAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1080-1086 bp | 1.0 | 82.9 |
| trnT-UGU-trnL-UAA_p2 | GGAAAAGCCAGCTATCGGAA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 1088-1094 bp | 1.0 | 82.4 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1032-1038 bp | 1.0 | 82.3 |
| trnT-UGU-trnL-UAA_p4 | AAAGCCAGCTATCGGAATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1077-1083 bp | 1.0 | 82.2 |
| trnT-UGU-trnL-UAA_p5 | AAAGCCAGCTATCGGAATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1085-1091 bp | 1.0 | 81.6 |