Hotspot

rps14-psaB

LSC · 40366-40496 · 131 bp

Back to Cyphostemma

Pi 0.0229

MarkerSeek score 65.70

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 3
pi: 0.0229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CTCAACGAGGGAACTTTGCT	CATTGTGCAGGCAAGATTGG	50.0	50.0	58.1	58.0	347 bp	1.0	79.7
rps14-psaB_p2	CTCAACGAGGGAACTTTGCT	CCATTGTGCAGGCAAGATTG	50.0	50.0	58.1	58.0	348 bp	1.0	79.7
rps14-psaB_p3	CTCAACGAGGGAACTTTGCT	TCTTGATTGCCTCCACATCG	50.0	50.0	58.1	58.0	279 bp	1.0	79.7
rps14-psaB_p4	CTCAACGAGGGAACTTTGCT	TCCATTGTGCAGGCAAGATT	50.0	45.0	58.1	58.1	349 bp	1.0	79.2
rps14-psaB_p5	CTCAACGAGGGAACTTTGCT	TTCCATTGTGCAGGCAAGAT	50.0	45.0	58.1	58.1	350 bp	1.0	79.2