Hotspot

psbZ-trnfM-CAU

LSC · 39021-39821 · 801 bp

Back to Cyphostemma

Pi 0.0191
MarkerSeek score 57.70
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
801
variable_sites
15
pi
0.0191
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 CAAGACCGGAGCTATCAACC CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.1 58.0 1425-1433 bp 1.0 81.4
psbZ-trnfM-CAU_p2 CAAGACCGGAGCTATCAACC ACAGATATGGTAGAGGGGCC 55.0 55.0 58.1 58.0 1530-1538 bp 1.0 81.3
psbZ-trnfM-CAU_p3 GATGCATTGTCCAACATGCC ACAGATATGGTAGAGGGGCC 50.0 55.0 58.1 58.0 1347-1350 bp 1.0 81.2
psbZ-trnfM-CAU_p4 CAAGACCGGAGCTATCAACC TCTTGATTGCCTCCACATCG 55.0 50.0 58.1 58.0 2101-2109 bp 1.0 81.0
psbZ-trnfM-CAU_p5 GATGCATTGTCCAACATGCC TCTTGATTGCCTCCACATCG 50.0 50.0 58.1 58.0 1918-1921 bp 1.0 80.9