Hotspot

trnY-GUA-trnE-UUC

LSC · 33551-33596 · 46 bp

Back to Cyphostemma

Pi 0.0217

MarkerSeek score 65.00

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 46
variable_sites: 1
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	189 bp	1.0	77.7
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	190 bp	1.0	77.7
trnY-GUA-trnE-UUC_p3	AGTGAATTATTGGGCCGAGC	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.3	57.9	219 bp	1.0	77.0
trnY-GUA-trnE-UUC_p4	GTGAATTATTGGGCCGAGCT	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.3	57.9	218 bp	1.0	77.0
trnY-GUA-trnE-UUC_p5	TGAATTATTGGGCCGAGCTG	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.3	57.9	217 bp	1.0	77.0