Hotspot

trnL-CAA

IRb · 116869-116949 · 81 bp

Back to Cyperus

Pi 0.117

MarkerSeek score 61.80

Primer available yes

Label trnL-CAA

Feature Metadata

label_name: trnL-CAA
feature_type: tRNA
region: IRb
length_bp: 81
variable_sites: 38
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA_p1	TGTTCCGCCCAAAATGAGAA	GTGTGGGGAGGATGATTTCC	45.0	55.0	58.0	57.9	1018-2689 bp	0.875	74.8
trnL-CAA_p2	TGTTCCGCCCAAAATGAGAA	TGTTTGAACAGCGTCAGTCA	45.0	45.0	58.0	58.0	1282-2459 bp	0.625	65.3
trnL-CAA_p3	TGTTCCGCCCAAAATGAGAA	TCCATAACCGCAAGTGCTAC	45.0	50.0	58.0	58.0	1329-2506 bp	0.625	65.2
trnL-CAA_p4	TGTTCCGCCCAAAATGAGAA	ATAACCGCAAGTGCTACAGG	45.0	50.0	58.0	58.0	1326-2503 bp	0.625	65.2
trnL-CAA_p5	TGTTCCGCCCAAAATGAGAA	AGCGTCAGTCAAACCTGTTT	45.0	45.0	58.0	58.0	1273-2450 bp	0.625	65.1