Hotspot

trnN-GUU-rps15

IRb · 128965-132020 · 3056 bp

Back to Cyperus

Pi 0.128

MarkerSeek score 80.40

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 3056
variable_sites: 899
pi: 0.128
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	CGGGTTCAGGGAGGTAAATG	ACATTTCGTCCCCAAGAACC	55.0	50.0	58.0	58.1	1076-1093 bp	1.0	90.9
trnN-GUU-rps15_p2	GATGGGGAACTCGCTTTAGG	ACATTTCGTCCCCAAGAACC	55.0	50.0	58.0	58.1	1013-1030 bp	1.0	90.9
trnN-GUU-rps15_p3	GTTCAGGGAGGTAAATGCGT	ACATTTCGTCCCCAAGAACC	50.0	50.0	57.9	58.1	1073-1090 bp	1.0	90.4
trnN-GUU-rps15_p4	GCTTATATGCCATGGGAGGG	ACATTTCGTCCCCAAGAACC	55.0	50.0	57.9	58.1	977-994 bp	1.0	90.2
trnN-GUU-rps15_p5	CGGGTTCAGGGAGGTAAATG	CATTTCGTCCCCAAGAACCT	55.0	50.0	58.0	57.8	1075-1092 bp	1.0	90.1