Hotspot

trnR-ACG-trnN-GUU

IRb · 126674-128892 · 2219 bp

Back to Cyperus

Pi 0.116

MarkerSeek score 77.60

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 2219
variable_sites: 602
pi: 0.116
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	TTCCTTTTTGAGGGTGCTCC	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	2406-2538 bp	0.75	71.3
trnR-ACG-trnN-GUU_p2	TAACTCGACGCCAGAATGTG	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	2703-2835 bp	0.75	71.1
trnR-ACG-trnN-GUU_p3	TTCCTTTTTGAGGGTGCTCC	AATGAGACATGGAAGGGGGA	50.0	50.0	58.0	58.0	2521-2653 bp	0.75	71.1
trnR-ACG-trnN-GUU_p4	CGAAATCACATGGCTGAGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	2430-2562 bp	0.75	71.1
trnR-ACG-trnN-GUU_p5	TAACTCGACGCCAGAATGTG	AATGAGACATGGAAGGGGGA	50.0	50.0	58.0	58.0	2818-2950 bp	0.75	71.0